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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurochem. 2011 Aug 8;118(6):1087–1100. doi: 10.1111/j.1471-4159.2011.07386.x

Fig. 5. Wild-type THAP1 interacts with the deletion mutant, THAP1Δ167-213.

Fig. 5

A) Schematic depicting a truncated THAP1 mutant which was generated to define residues required for THAP1 to bind itself. (B) THAP1Δ167–213-FLAG in U2OS cells was localized in both nucleus and cytoplasm, similar to wtTHAP1, although some cells were observed in which the transgene appeared to accumulate in the perinuclear region (arrows). Images captured at 100X magnification; scale bar = 10 μM. (C) V5-wtTHAP1 immunoprecipitated the FLAG-tagged deletion mutant with similar efficiency as it did wtTHAP1-FLAG, indicating that residues beyond position 166 are not necessary for THAP1 to bind itself.