Intrathecal administration of 1 μg LPS + prior administration of subcutaneous CORT (2.5 mg/kg) or vehicle. IL-1ra (100 μg in 1 μl) or equivolume vehicle was coadministered intrathecally with the LPS. Mechanical sensitivity was tested using the von Frey test, before (baseline, BL) CORT or vehicle, before (0) and 1–24 h after intrathecal administration. Time is reflected as post intrathecal administration. Solid circle depicts CORT+LPS+vehicle(n= 6), black squares depict vehicle+LPS+IL-1ra (n=6) and open circles depicts CORT+LPS+IL-1ra (n= 6). Data are presented as average absolute thresholds of left and right hind paws (mean ± SEM).
***P< 0.001, against CORT+LPS+vehicle.