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. 1999 Apr 15;13(8):946–953. doi: 10.1101/gad.13.8.946

Figure 2.

Figure 2

The EXD/PBX1 and HTH/PREP1 proteins are mutually required for nuclear localization in transfected Drosophila Schneider cells. A subregion of the PBC-A domain is necessary for cytoplasmic localization of PBX1 in Schneider cells. Drosophila Schneider cells were transiently transfected with expression constructs for the indicated proteins, and processed for indirect immunofluorescence with anti-PREP1 and anti-PBX1 polyclonal, or anti-FLAG and anti-HA monoclonal antibodies. PBX1 (A, green) and PREP1 (B, red) are found in the cytoplasm of expressing Schneider cells. Coexpression of PREP1 with PBX1 (C, green) causes nuclear localization of PREP1 (D, red). A PBX1 mutant, representing a deletion of its amino-terminal PBC-A domain, PBXΔ1–140 (E, green), is nuclear in Schneider cells in the absence of PREP1 or HTH. In contrast, the PBXΔ1–72 mutant (F, red) is found in the cytoplasm. PBXΔ1–90 is nuclear in expressing cells (G, green). Double stainings (C,D) were performed with anti-HA (rat) monoclonal, and anti-PREP1 polyclonal antibodies.