Figure 3.

LMB blocks nuclear export of PBX1, EXD, and PBXΔ1–72 proteins in Drosophila Schneider cells. Cells were transiently transfected with expression constructs for PBX1, EXD, and PBXΔ1–72 and processed for indirect immunofluorescence with anti-PBX1 and anti-Flag antibodies. Schneider cells, transfected with the PBX1, the EXD or the PBXΔ1–72 expressors were treated with 5, 50, and 250 nm LMB as indicated. Fifty and 250 nm LMB induce relocalization of PBX1 (A, green), of EXD (B, red), or of the PBXΔ1–72 mutant (C, red) into the nuclei of expressing cells. Treatment with LMB at the indicated concentrations was performed 18 hr after transfection.