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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Proteome Res. 2011 Jul 26;10(9):4005–4017. doi: 10.1021/pr2002098

Figure 2.

Figure 2

Comparison of UPLC gradients for LC-MRM assays. XIC of MRM transitions for the enolase peptide YDLDFK from the 73 and 20 min gradients, and a 20 min gradient with scheduled MRM enabled. For scheduled MRM, the detection window was set at 2 min, and target scan time was 1 s. The intensities, areas, and peak widths of the most intense y5 transition (400.69/637.32, black line) are indicated. Other transitions are y4 (400.69/522.29, green line), y3 (400.69/409.21, blue line), and b4 (400.69/507.21, red line). The time axes are shown using the same scale.