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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Proteome Res. 2011 Jul 26;10(9):4005–4017. doi: 10.1021/pr2002098

Figure 5.

Figure 5

Reproducibility of the label-free MRM analysis using major protein depletion and 1-D SDS fractionation. MRM data for ADAM12, PAEP, and CGB are shown. Three peptides for each protein were quantitated. Depletions 1 and 2 are replicate Top-20 depletions of EP and IUP serum samples performed on different days. Run 1 and Run 2 are duplicate injections of tryptic digests onto a 4000 QTRAP mass spectrometer. Bars indicate areas of the strongest MRM transition for each peptide, summed for gel slices 12–15. The % CV of all replicate runs is indicated above each sample.