Table 1.
Reproducibility of MRM Quantitation of 10 Femtomol Enolase Standard Peptides
| Sequence | Q1 m/z | Q3 m/z | Retention Time (Min)a | Intensity (cps)a | Area (counts)a | %CVb |
|---|---|---|---|---|---|---|
| SVYDSR | 363.67 | 540.24 | 17.3±0.1 | 5.88E+04 | 4.60E+05 | 21.5 |
| GVLHAVK | 362.23 | 567.36 | 17.6±0.1 | 3.05E+03 | 2.51E+04 | 10.5 |
| ANIDVK | 330.19 | 361.21 | 17.6±0.1 | 2.32E+04 | 1.90E+05 | 12.9 |
| HLADLSK | 392.22 | 437.21 | 17.6±0.1 | 2.01E+04 | 1.64E+05 | 14.8 |
| IATAIEK | 373.23 | 561.32 | 18.0±0.1 | 5.74E+04 | 4.55E+05 | 11.6 |
| IGSEVYHNLK | 580.31 | 674.36 | 19.0±0.1 | 5.30E+03 | 4.14E+04 | 14.4 |
| NVPLYK | 367.22 | 520.31 | 19.5±0.1 | 1.01E+05 | 8.94E+05 | 18.4 |
| LNQLLR | 378.74 | 401.29 | 20.4±0.1 | 8.20E+04 | 6.30E+05 | 11.0 |
| TFAEALR | 404.22 | 559.32 | 20.9±0.1 | 2.40E+05 | 1.69E+06 | 12.1 |
| GNPTVEVELTTEK | 708.86 | 948.49 | 21.3±0.1 | 9.34E+03 | 6.88E+04 | 11.8 |
| VNQIGTLSESIK | 644.86 | 834.46 | 21.7±0.1 | 6.44E+04 | 4.49E+05 | 10.3 |
| AADALLLK | 407.76 | 743.47 | 22.0±0.1 | 4.71E+04 | 3.45E+05 | 15.6 |
| YDLDFK | 400.69 | 637.32 | 22.2±0.1 | 9.94E+04 | 7.39E+05 | 12.9 |
| NVNDVIAPAFVK | 643.86 | 1073.60 | 23.3±0.1 | 2.82E+04 | 2.19E+05 | 15.3 |
| TAGIQIVADDLTVTNPK | 878.48 | 1073.55 | 24.0±0.1 | 3.24E+03 | 2.21E+04 | 29.5 |
| AVDDFLISLDGTANK | 789.90 | 918.49 | 25.7±0.1 | 1.72E+03 | 1.19E+04 | 32.3 |
a
Average values from 22 enolase standard runs spread over a total of 206 MRM analyses spanning 12 days. Peptides with average intensities less than 200 cps were excluded from this table. Cps, counts/second.
b
The coefficients of variation were determined from the peak area for the peptides in the 22 standard runs.