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. 1999 Apr 15;13(8):1025–1036. doi: 10.1101/gad.13.8.1025

Figure 7.

Figure 7

Auto-down-regulation of the ΔCbfa1 transgene and transient aspect of the osteopenic phenotype in ΔCbfa1-expressing mice. (A) Functional organization of the 1.3-kb OG2 promoter. The OSE1 site and the two OSE2 sites are indicated by open and solid boxes, respectively. (B) Histological analysis of long bones (tibiae) of 2-, 4-, and 8-week-old wild-type and ΔCbfa1-expressing mice. Sections through the metaphyses stained with hematoxylin/eosin. The amount of trabecular bone (pink, arrows) is severely decreased in 2-week-old trangenic mice and return to normal in 8-week-old animals. (C) RT–PCR analysis of ECM protein-encoding gene expression in long bones of 4- and 8-week-old wild-type and ΔCbfa1-expressing mice. Expression of Osteocalcin, Bone sialo protein, Osteopontin, α1(I)collagen, and α2(I)collagen in the transgenic mice is still decreased in 4-week-old animals, but less than that in 2-week-old mice (cf. with Fig. 6A), and returns to normal in 8-week-old mice. Amplification of exon 2 of Hprt was used as an internal control for cDNA quality and PCR efficiency in each lane. (D) Temporal analysis of ΔCbfa1 expression in transgenic animals. Expression of the transgene peaks at 2 weeks of age and is decreased severely thereafter. Amplification of exon 2 of Hprt was used as an internal control for cDNA quality and PCR efficiency in each lane.