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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurochem. 2011 Aug 8;118(6):1113–1123. doi: 10.1111/j.1471-4159.2011.07383.x

Fig 7.

Fig 7

Apelin-induced phosphorylation of NR2B S1480 mediates protection against excitotoxicity in HEK293 cells. (a) Cultures transfected with APJ, NR1, PSD-95, GFP, and wild-type (WT) NR2B or NR2B S1480A-expressing plasmids were treated with apelin-36 (20μM) for 5min, followed by NMDA (50μM) + glycine (10μM) for 5min. Phosphorylation of NR2B at S1480 was assessed by Western blot (n = 3). (b) Cultures were treated with apelin-36 (0.2, 20μM), apelin-36 scramble (20μM), or MK801 (10μM) for 45min, followed by glutamate (50μM) for 24hr. Transfected cell survival was quantified by counting GFP-positive cells and expressed as the ratio of GFP-positive cells to the total number of cells (n = 5–6 coverslips per treatment condition, 25–30 fields total; *p<0.05, **p<0.01, ***p<0.001 vs. vehicle by one-way ANOVA plus Newman-Keuls post hoc testing).