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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2011 Jul 29;68(8):446–458. doi: 10.1002/cm.20523

Figure 8.

Figure 8

Centrosome amplification in G1/S-arrested cells by expression of exogenous Plk4. A: Histograms of the average numbers of centrosomes counted in mock transfected (blue), and transfected cells expressing (yellow) and non-expressing (brown) GFP-tagged chicken Plk4 during PP1 treatment. B: Cells expressing and non-expressing GFP-Plk4 (B2) were stained with γ-tubulin antibody (B1). After treatment with 5 μM PP1 for 22 hr, cells were transfected with GFP-Plk4 and further cultured prior to fixation at 48 and 68 hr. A merged image with DAPI-staining is shown in B3. Only cells expressing GFP-Plk4 amplified centrosomes. Bar, 10 μm.