Human papillary cells were grown on glass coverslips for 3–4 d in the presence (+) or absence (−) of serum or in serum-free medium + EGF as indicated. Fixed cells were immunostained with antibodies against cell junction proteins (adherens junctions: E-cadherin, N-cadherin, pan-cadherin and β-catenin; tight junctions: occludin), the neural stem cell marker nestin, or beta III tubulin, a specific marker for immature neurons. Scale bars 20 µm.