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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jan 19;1812(10):1344–1357. doi: 10.1016/j.bbadis.2011.01.010

Figure 6. Human CD133/1+ adult renal stem cells incorporate within tubules of metanephric organ cultures.

Figure 6

(A) Kidneys were harvested from E12.5 FVB/n mice and (B) injected with 1500cells/site CD133/1+ human kidney cells from the papilla or cortex using capillary pipets with 40 µm tips. Three individual kidneys were injected with each cell type. (B) Human cells were prelabeled with fluorescent CFDA to allow tracking (CFDA, pseudocolored red). Kidney cultures were incubated in DMEM/F12 for three days post-injection, fixed, stained with fluorescent peanut agglutinin (pseudocolored green) and imaged with a two-photon microscope. Arrows denote cells integrated into tubules. See supplemental movie 2 for view through entire Z-stack.