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Type III secretion system-encoding gene expression in Echerichia coli strains carrying a virulence plasmid from Shigella sonnei. Immunoblot analysis of IpaB and InvE expression at 37 °C. A nonspecific protein that cross-reacted with the InvE antibody (lower panel) was used as a loading control throughout the study. Lanes: 1, HW1273 (WT); 2, ME2824 (ΔcpxA); 3, ME5201 (ΔrodZ); 4, ME5199 (ΔcpxA/ΔrodZ). WT, wild type.
