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. 2011 Jul 22;12(9):911–916. doi: 10.1038/embor.2011.132

Figure 5.

Figure 5

Functional domain mapping. (A) Immunoblot analysis of InvE protein at 30 °C in MS5201 (ΔrodZ) strains carrying the indicated expression plasmids. Lanes: 1, WT MS390; 2, ΔrodZ strain carrying pBAD-rodZ; 3, VC; 4, ΔKRRKKR; 5, R62G; 6, R70G; 7, R90G; 8, R62G, R70G; 9, R62G, R90G; 10, R70G, R90G; 11, R62G, R70G, R90G; 12, R62G, R70G, R90G with KRRKKR (Rev.). (B) Phase contrast images of strain MS5201 (ΔrodZ) expressing the indicated RodZ variants. Scale bars, 10 μm. Rev., reversion of the short basic region; VC, vector control; WT, wild type.