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Dominant-negative TRAF2 can potentiate TNFR1-mediated death. 293 cells were transfected with 100 ng of GFP plasmid, together with 1 μg of the indicated plasmids, and either mock treated or treated with human TNF-α (200 U/ml), and cell viability was determined as described in the legend to Fig. 1 and in Materials and Methods.
