Tm curves (A) and CD spectra (B) of homoadenine acpcPNA·DNA hybrids at different ratio. Conditions: (A) 1.0 µM acpcPNA, 10 mM sodium phosphate buffer pH 7.0, 0–500 mM NaCl, heating rate 1.0°C/min; (B) 2.0 µM acpcPNA, 10 mM sodium phosphate buffer pH 7.0, 0 mM NaCl, 25°C.