Table 2.
Tm data for the hybrids between acpcPNA T9 and A9 with complementary and single mismatched DNA
| Entry | PNA | DNA | Tm (°C)a,b | ΔTm (°C)c | |
| 1 | T9 | dAAAAXAAAA | X = A | 76.8 | - |
| 2 | X = T | 47.6 | −29.2 | ||
| 3 | X = C | 47.7 | −29.1 | ||
| 4 | X = G | 39.0 | −37.8 | ||
| 5 | A9 | dTTTTYTTTT | Y = A | 54.6 | −25.3 |
| 6 | Y = T | 79.9 | - | ||
| 7 | Y = C | 56.5 | −23.4 | ||
| 8 | Y = G | 52.7 | −27.2 | ||
a
Conditions: 1.0 µM acpcPNA, 1.0 µM DNA, 10 mM sodium phosphate buffer pH 7.0, 0 mM NaCl, heating rate 1.0°C/min. Tm values are accurate to within ±0.5°C.
b
Data for T9 (entries 1–4) were taken from reference 33.
c
Tm (mismatched) − Tm (complementary).