Table 4.
Comparison of Tm data to show specificity of binding between mixed base acpcPNA M10a and complementary/single base mismatched DNA
| Entry | DNA sequence (5′ → 3′) | Tm (°C)a | ΔTm (°C)b | Note |
| 1 | AGT GAT CTA C | 53.3c | - | complementary |
| 2 | AGT AAT CTA C | 26.4 | −26.9 | pC·dA mismatch |
| 3 | AGT TAT CTA C | 27.8 | −25.5 | pC·dT mismatch |
| 4 | AGT CAT CTA C | 31.0 | −22.3 | pC·dC mismatch |
| 5 | AGT GGT CTA C | 23.9c | −29.4 | pT·dG mismatch |
| 6 | AGT GTT CTA C | 29.4c | −23.9 | pT·dT mismatch |
| 7 | AGT GCT CTA C | 23.8c | −29.5 | pT·dC mismatch |
| 8 | AGT GAG CTA C | 26.5 | −26.8 | pA·dG mismatch |
| 9 | AGT GAA CTA C | 28.4 | −24.9 | pA·dA mismatch |
| 10 | AGT GAC CTA C | 28.8 | −24.5 | pA·dC mismatch |
| 11 | AGT GAT GTA C | 26.4 | −26.9 | pG·dG mismatch |
| 12 | AGT GAT ATA C | 24.2 | −29.1 | pG·dA mismatch |
| 13 | AGT GAT TTA C | 28.5 | −24.8 | pG·dT mismatch |
a
Conditions: 1.0 µM acpcPNA, 1.0 µM DNA, 10 mM sodium phosphate buffer pH 7.0, 100 mM NaCl, heating rate 1.0°C/min. Tm values are accurate to within ±0.5°C.
b
Tm (mismatched) − Tm (complementary).
c
Data taken from reference 36.