Table 5.

T_m data for the binding between mixed base acpcPNA and RNA in antiparallel and parallel directions^a

Entry	PNA	%G+C	Tm (antiparallel RNA) (°C)	Tm (parallel RNA) (°C)
1	M10a	40	42.3b	<20b
2	M10b	70	48.0	32.9
3	M10c	20	32.6	<20
4	M10d	40	40.8	not determined
5	M10e	40	40.9	not determined
6	M10f	70	49.3	30.2
7	M10g	70	51.2	28.5
8	M10h	20	39.7	not determined
9	M10i	20	37.7	not determined

^aConditions: 1.0 µM acpcPNA, 1.0 µM DNA, 10 mM sodium phosphate buffer pH 7.0, 100 mM NaCl, heating rate 1.0°C/min. T_m values are accurate to within ±0.5°C.

^bData taken from reference ³⁶.