Table 6.

Thermodynamic parameters of hybrids of acpcPNA with DNA and RNA^a

Entry	PNA	Counterstrandb	%G+C	Tm (°C)c	ΔH° (kcal/mol)c	ΔS° (cal/mol·K)c	ΔG°298 (kcal/mol)c
1	M10a	DNA	40	53.1	−64.1 ± 1.0 (−64.1 ± 0.7)	−168 ± 3 (−167 ± 2)	−14.1 ± 0.1 (−14.1 ± 0.0)
2	M10b	DNA	70	56.4	−68.9 ± 1.9 (−69.0 ± 3)	−180 ± 6 (−180 ± 10)	−15.2 ± 0.3 (−15.2 ± 0.4)
3	M10c	DNA	20	53.8	−60.9 ± 1.0 (−61.7 ± 1.0)	−157 ± 3 (−160 ± 3)	−14.0 ± 0.0 (−14.1 ± 0.0)
4	M10a	RNA	40	42.8	−60.0 ± 0.2 (−60.1 ± 0.9)	−161 ± 0.5 (−161 ± 3)	−12.0 ± 0.1 (−12.0 ± 0.0)
5	M10b	RNA	70	48.1	−68.0 ± 2.6 (−67.9 ± 2.8)	−183 ± 8 (−183 ± 8)	−13.5 ± 0.3 (−13.5 ± 0.3)
6	M10c	RNA	20	33.1	−54.9 ± 3.1 (−54.3 ± 1.4)	−151 ± 10 (−148 ± 4)	−10.1 ± 0.3 (−10.0 ± 0.2)

^aConditions: 1.0 µM of each acpcPNA and DNA or RNA strand, 10 mM sodium phosphate buffer pH 7.0, 150 mM NaCl, heating rate 1.0°C/min.

^bAntiparallel complementary DNA or RNA.

^cT_m and thermodynamic parameters are obtained from curve fitting.⁴⁴ Those obtained from van't Hoff analysis⁴⁵ are shown in parentheses. All figures are average of at least three independent analyses. Errors are calculated based on standard deviation.