Figure 5.
Dose dependent detection of trypanosoma18S rRNA. The PNA beads (red beads (RB) with PNA3534 and green beads (GB) with PNA3533), were incubated with the indicated amounts of (A) in vitro transcribed target 18S RNA or (B) total trypanosome RNA, and subjected to fluorescence microscopy analysis (×250 for top raw and ×400 for other raws) after magnetic enrichment. RBs in co-localization with GB(s) are circled with a dotted red line where GBs with co-localization are indicated by yellow arrows.