Figure 2.

Distinct effects of EGF and TGFβ1 on pancreatic cancer cells in 3D and soft agar culture. PCCs were seeded in 3% Matrigel™/soft agar (3D) or in 0.4% soft agar in parallel experiments. (A) Four days after seeding, cells were treated with 100 pM TGFβ1 and/or 1 nM EGF as indicated. Cells were stained with MTT 8 days after treatment and images were captured by highresolution scanning. Scale bars indicate image magnification. (B Colorimetric intensity of MTT signal was quantified; average intensity value and standard deviation are displayed for each sample (n = 3) of a representative experiment. Relative fold change (over +/−2X) and statistical significance of this change with respect to EGF/TGFβ1-treated samples are displayed on top of the sd bars.