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Transmission of Sup35 protein to daughter cells is conformation-dependent. (left) Schematic of fluorescence loss in photobleaching assay (FLIP) for Sup35 transmission to daughter cells. Bleached daughter (red) and monitored mother (black) are indicated. (right) Fluorescence retention in mother cells expressing Sup35-GFP in the [PSI+]strong (white), [PSI+]weak (pink) or [psi−] (red) conformation.
