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Schematic features of miR cassettes and knockdown efficacy in N2a cells. (A) Each miR cassette in pcDNA6.2-GW/EmGFP vector. (B) Western blot of N2a cells transfected with each miR expression construct. (C) Quantitation of PrPC expression. When miRdual was introduced into the cells, PrPC expression was the most efficiently decreased as compared with wild-type cells (*p < 0.05, **P <0.01).
