Skip to main content
. 2011 Apr-Jun;5(2):93–102. doi: 10.4161/pri.5.2.15621

Figure 2.

Figure 2

Transfection of miR cassettes and Prnp knockdown efficacy at mRNA and protein level. (A) PCR of genomic DNA shows miR cassette-specific amplicons. Lane L, 100 bp DNA ladder; lane 1, expression vector with miRdual cassette; lane 2, expression vector with miRscr; lane 3, wild-type cells; lane 4, N2amiRdual; lane 5, N2amiRscr; lane 6, no template. (B) qPCR analysis of PrPC transcript abundance showing knockdown efficiency of miRdual in mRNA levels. (C) Western blot analysis of PrPC protein abundance. Top part is the western blot and bottom panel is densitometric evaluation. dual1 and dual2 are N2amiRdual cell clones (*p < 0.05, **p < 0.01).