Table 1.

Target genes of qPCR for characterizing the established N2amiRdual cell line

Gene Symbol	Gene Name	GenBank Accession No.	General Putative Function	Ref.
Prnp	prion protein	NM_011170	The function of PrPC is not known. PrPC is encoded in the host genome and is expressed both in normal and infected cells.
6720460 F02Rik	RIKEN cDNA 6720460F02 gene	AK049710	Unknown	30
Cdr34	Cerebellar degeneration-related antigen 1	NM_001166658	In fission yeast, acts as a mitotic inducer. In G2 it negatively regulates wee1, a mitotic inhibitor. Also has a role in cytokinesis where it required for proper septum formation.	31
Csnk2a1	casein kinase 2, alpha1 polypeptide	AK011501	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha′ chains contain the catalytic site.	32
Gfap	Glial fibrillary acidic protein	AF332061	GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.	33
Mpg	N-methylpurine-DNA glycosylase	NM_010822	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.	30
Plk3	Polo-like kinase 3 (Drosophila)	NM_013807	Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion.	30
Ppp2r2b	protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform	NM_028392	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.	31