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. 2011 Apr-Jun;5(2):93–102. doi: 10.4161/pri.5.2.15621

Table 3.

Primer sequences used in this study

Target Gene Purpose Label Sequence (5′ to 3′) Size Product Size
miR cassette PCR - F TGC TGC TGC CCG ACA ACC ACT ACC TGA GCA 30 412
- R ATC AGC GAA CCG CGG GCC CTC TAG ATC AAC 30
6720460 F02Rik PCR - F GAT CAC TCC TAA AGG AGG AAC AGC TAG AG 29 643
- R ATA AGG GAG ACG GTC ATG TCA CCA C 25
qPCR FAM F CGT TAC TCA GCC TGA ACC TCG GTA A 25 68
- R CTG CTC TGG CTC TGG ACT TTC C 22
Cdr34 PCR - F ATG CTG GCA GAT AAC CTA GTG GAG G 25 1,240
- R AAC TGA TGG ACT CTG GGC TTA CAG G 25
qPCR FAM F CGT GGA GAG CTA CTG AAG AAG TGC CA 26 67
- R AGG TCT GCA CAG CCT TGT GTG 21
Csnk2a1 PCR - F AAG CAG GGC CAG AGT TTA CAC AGA T 25 1,137
- R GCT GGA ACA GGT ATC CCA AGT GAG T 25
qPCR FAM F CGA AGT TTC TGG ACA AGC TGC TT 23 104
- R AGC CTG GTC CTT CAC AAC AGT G 22
Gfap PCR - F ACG CTT CTC CTT GTC TCG AAT GAC T 25 1,178
- R GCT CCT GCT TCG AGT CCT TAA TGA C 25
qPCR FAM F CGG ATA CTT TCT CCA ACC TCC AGA TC 26 77
- R CCT CTT GAG GTG GCC TTC TGA C 22
Mpg PCR - F GGG CAG AGG ATC CCT AAA ACC GGT G 25 942
- R CAG GCT GTT TGC TGA GGC TGA TCC A 25
qPCR FAM F CGA CAG CCC TAA AGA GAG ACT CCT GT 26 74
- R GGT CCT CTG GGC TGG AGA AGT 21
Plk3 PCR - F CTA TGA AGC CAC TGA CAC CGA GTC T 25 1,629
- R GAA GCG AGG TAA GTA CAA GCA CTG C 25
qPCR - F CTT GGT GAG TGG CCT CAT GC 20 75
FAM R CGG AGT GAA ACT ACA GGA GCC TC 23
Prnp PCR - F CTG CTG GCC CTC TTT GTG ACT ATG T 25 736
- R CGA TCA GGA AGA TGA GGA AGG AGA T 25
qPCR FAM F CGA AGC AGC AAC CAG AAC AAC TT 23 65
- R ACC GTG TGC TGC TTG ATG GT 20
Ppp2r2b PCR - F AGG AGG ACA TTG ATA CCC GCA AAA T 25 1,273
- R ATG TTT TCT GAA GGA TGC CAA GCT G 25
qPCR FAM F CGG TCT TCT TCA GGA TGT TCG AC 23 191
- R AGG ATG CCA AGC TGT ATG CAA G 22