Skip to main content
. Author manuscript; available in PMC: 2011 Sep 4.
Published in final edited form as: Peptides. 2009 Nov 26;31(2):263–270. doi: 10.1016/j.peptides.2009.11.015

Fig. 5.

Fig. 5

Abdominal surgery induces Fos expression in nesfatin-1 immunopositive neurons in the rat rostral raphe pallidus (rRPa), nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM). Double immunohistochemical staining for Fos (dark blue) and nesfatin-1 (brown) 120 min after abdominal surgery in the rRPa (B), NTS (E), VLM (H) and in control rats (A, D, G). The inserts in B, E and H show higher magnification of activated neurons co-localizing with nesfatin-1. Scale bar: 100 μm in A, B, D E, G, H and 10 μm in the inserts. Cell count per section (unilateral) in the rRPa (C), NTS at the level of the area postrema (F) and VLM (I). Data are mean ± SEM of 6 rats/group. * p < 0.05; ** p < 0.01. AP = area postrema; CC = central canal; DMN = dorsal motor nucleus of vagus; py = pyramidal tract.