| Annealing Reaction | |
| Nuclease free water | 7.3 μl |
| 1 – 10 oocytes or embryos in M2 medium | 1.0 μl |
| 5% NP-40 | 2.0 μl |
| T7 Promoter Primer 8-fold dilution | 1.2 μl |
| Total Volume | 11.5 μl |
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