| cDNA Reaction | |
| Annealing Reaction from above | 11.5 μl |
| 5X First Strand Buffer | 4.0 μl |
| 0.1 M DTT | 2.0 μl |
| dNTP Mix (10mM) | 1.0 μl |
| MMLV RT | 1.0 μl |
| RnNase Out | 0.5 μl |
| Total Volume | 20.0 μl |
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