| cDNA reaction from above | 20.0 μl |
| Nuclease free water | 12.1 μl |
| 4X Transcription Buffer | 20.0 μl |
| 0.1 M DTT | 6.0 μl |
| NTP Mix | 8.0 μl |
| CTP | 5.6 μl |
| 50% PEG | 6.4 μl |
| RnNase Out | 0.5 μl |
| Inorganic Phosphate | 0.6 μl |
| T7 RNA Polymerase | 0.8 μl |
| Total Volume | 80.0 μl |
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