| cDNA Reaction - Second Strand | |
| Annealing Reaction from above | 21.2 μl |
| Nuclease free water | 5.8 μl |
| 5X First Strand Buffer | 4.0 μl |
| 0.1 M DTT | 4.0 μl |
| dNTP Mix (10mM) | 2.0 μl |
| MMLV RT | 2.0 μl |
| RnNase Out | 1.0 μl |
| Total Volume | 40.0 μl |
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