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. 2000 Jun 1;14(11):1353–1363.

Figure 3.

Figure 3

CLD and NLS of mPER3 protein. Three truncated mutants of mPER3 fused with GFP were expressed in COS7 cells. (A) Schematic diagrams of the five constructs; (1) mPER3 (total), (2) mPER3 (ΔCLD), (3) mPER3 (228–1077) containing PAS-B, CLD, and NLS, (4) mPER3 (324–1077) containing CLD and NLS, and (5) mPER3 (542-1077) containing NLS and carboxy-terminal portion. (B) GFP fusion proteins analyzed by immunoblot confirmed the expression of expected GFP fusion proteins. (C) Example photos demonstrating the subcellular localization of each construct. GFP fusion proteins containing both the CLD and NLS [mPER3 (228–1077) and mPER3 (542–1077)] were localized only in the cytoplasm. Deletion of only the CLD region of mPER3 (ΔCLD) greatly increased the nuclear signals. (D) Summary of deletion study using truncated mutants of mPER3. Each bar indicates the percentage of cells expressing GFP-fusion proteins with predominant nuclear staining. All data were mean (+s.e.m.) in three independent experiments. In each experiment, 10 fields containing ∼200 cells altogether were examined.