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. Author manuscript; available in PMC: 2011 Sep 5.
Published in final edited form as: Methods Enzymol. 2010;482:35–72. doi: 10.1016/S0076-6879(10)82002-6

Fig.1.

Fig.1

Biological particle suspended in a layer of amorphous ice with an optional thin carbon layer support. Depending on the specimen, the size h of the particle is between fifty and few thousand Ångstroms. The ice thickness H should not exceed the particle size by much.