Figure 5.

DEK inhibits the replication of chromatin but not of protein-free DNA. (A) SV40 DNA and SV40 chromatin were preincubated with DEK and topoisomerase I and used as templates in the SV40 in vitro replication system. Purified DNA was separated on an agarose gel and visualized by ethidium bromide staining. (B) Autoradiography of the agarose gel shown in A. The position of replicative intermediates (R.I.) of supercoiled (I) and relaxed closed circular and nicked molecules (II) are indicated. (C) Nucleotide incorporation (pmole) of the samples shown in B, as determined by TCA precipitation.