Figure 3.
The effects of sauchinone (Sau) on hepatic glutathione (GSH) contents and glutamate-cysteine ligase (GCL), NAD(P)H:quinone oxidoreductase-1 (NQO1) and heat shock protein 32 (HSP32) expression. (A) Hepatic GSH contents. Mice were treated with acetaminophen (APAP) or APAP + Sau, as described in the legend to Figure 1 (n = 6 each). (B) Real-time PCR assays for modifier subunit of glutamate-cysteine ligase (GCLM), NQO1 and HSP32. Sau (30 mg·kg−1·day−1) was administered to wild-type (WT) or nuclear factor erythroid-2-related factor-2 (Nrf2) knockout mice for 3 days, and the liver samples were collected 6 h after last injection of Sau (n = 6 each). (C) Immunoblottings for GCL, NQO1 and HSP32 in the liver homogenates. Values indicate scanning densitometric results. Representative data from six samples. (D) Immunoblottings for Nrf2 in the nuclear fractions of liver homogenates. Representative data from six samples.