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. 2011 Aug;163(8):1653–1665. doi: 10.1111/j.1476-5381.2010.01095.x

Figure 4.

Figure 4

Induction of glutamate-cysteine ligase (GCL) and NAD(P)H:quinone oxidoreductase-1 (NQO1) by sauchinone. (A) Real-time PCR assays. The expression of nuclear factor erythroid-2-related factor-2 (Nrf2) target genes was monitored in HepG2 cells using real-time PCR assays, in which the mRNA level of GAPDH was used as a reference for data normalization. Cells were treated with vehicle or 30 µM sauchinone (Sau) for 12 h and subjected to the preparation of mRNA, from which cDNA was synthesized by reverse transcriptase. Fold changes were calculated by correlation coefficients of crossing point (Cp) for triplicate PCR results. (B) Immunoblottings for GCL and NQO1. The proteins of interest were immunoblotted in the lysates of HepG2 cells that had been treated with vehicle or 30 µM sauchinone for 24 h. GCLC, catalytic subunit of glutamate-cysteine ligase; GCLM, modifier subunit of glutamate-cysteine ligase.