Figure 7.
Visualization of interdigital cell death by Nile Blue Sulphate vital staining. Limb buds were isolated at E13.5. Plantar (ventral) apects of hindfeet are shown. Apoptotic cells that stain blue with Nile Blue Sulphate are observed between all forming digits of wild-type and heterozygous littermates, as well as in the anterior (a) and posterior (p) necrotic zones (A) In Jag2ΔDSL homozygotes (B,C), the numbers of staining cells are greatly reduced in the regions where digits 2, 3, and 4 would normally form. There is no apparent reduction of staining cells in the region between digits 1 and 2, and in the anterior and posterior necrotic zones.