Table 1.
Summary of flow cytometry analyses of fetal thymocytes from Jag2ΔDSLmutant embryos and littermate controls
| Thymic subset analyzed |
Jag2 genotype
|
|
|---|---|---|
| +/+, +/−
|
−/−
|
|
| CD4−CD8− | 7.9 ± 0.9 (n = 14) | 8.0 ± 1.1 (n = 11) |
| CD4+CD8+ | 83.6 ± 1.2 (n = 14) | 85.6 ± 1.4 (n = 11) |
| CD4+CD8− | 3.2 ± 0.3 (n = 14) | 3.3 ± 0.3 (n = 11) |
| CD4−CD8+ | 4.3 ± 0.9 (n = 14) | 3.3 ± 0.9 (n = 11) |
| TCRαβ+ among CD4+CD8+ cells |
52.1 ± 1.0 (n = 19) | 50.5 ± 1.1 (n = 15) |
| TCRγδ+ among total thymocytes | 2.6 ± 0.1 (n = 19) | 1.3 ± 0.1 (n = 15)a |
| TCRγδ+ among CD4−CD8− cells |
19.6 ± 1.3 (n = 19) | 11.8 ± 1.0 (n = 15)a |
Total cells isolated per thymic lobe did not differ between mutants and controls. No differences were noted between +/+ and +/− littermate controls, so data from these two genotypes were combined. Data are presented as the mean % ± s.e.m. of cells expressing the indicated differentiation markers. Numbers of embryos tested are given in parentheses.
a
Significantly less (P < 0.001, Student’s t-test) than in +/+ and +/− littermate controls.