Figure 8.
The stf1.1 mutant has a temperature-sensitive spindle defect in a cdc25.22 background. (a–i) Immunofluorescence analysis of the cdc25.22 and stf1.1 single mutants and the double mutant cdc25.22 stf1.1. Cells were cultured in complete medium to mid-log phase at 25°C and then shifted to 37°C, and processed for immunofluorescence analysis. (a–c) Phase/DAPI; (d–f) anti-tubulin staining; (g–i) anti-Sad1 staining. (a,d,g) cdc25.22; (e,f,h) stf1.1; (c,f,i) cdc25.22 stf1.1. The single cdc25.22 mutant arrested with an interphase cytoskeleton characteristic of a G2 cell, whereas the stf1.1 mutant continued to form normal spindles and divide. The double-mutant cdc25.22 stf1.1 formed aberrant star-shaped spindles arising from a single large Sad1 focus within the cell. Scale bar, 10 μm.