Figure 1.

 The dU2AF⁵⁰ RS domain is inessential in vivo. (A) Wild-type (WT) and dU2AF⁵⁰ deletion and substitution derivative transgenes were tested for complementation of a dU2AF⁵⁰ recessive lethal allele. A schematic diagram of the dU2AF⁵⁰ domains is shown. The RS domain (RS), the dU2AF³⁸ interaction domain (int), and the three RNA recognition motifs (RRM) are indicated. The percentages of rescue are from representative transgene lines. (B) Amino acid sequence comparison of the RS domains from dU2AF⁵⁰ and hU2AF⁶⁵. Identities and similarities are shown in black and gray boxes, respectively. Dashes denote gaps. Amino acid positions are shown on the right. The caret below the sequence indicates the dU2AF⁵⁰ RS domain deletion site.