Figure 5.

 The dU2AF³⁸ RS domain is not required for enhancer-dependent dsx splicing in vivo. (A) RT–PCR analysis of dsx splicing. ³²P-labeled RT–PCR products were subjected to electrophoresis through a native polyacrylamide gel and visualized by autoradiography. Total RNA isolated from w¹¹¹⁸ (dU2AF³⁸⁺) males (lanes 1,2) or females (lanes 3,4) flies or from dU2AF³⁸ mutant males (lanes 5,6) or females (lanes 7,8) rescued by the dU2AF³⁸ΔRSGly transgene was analyzed. The presence or absence of reverse transcriptase (RT) in the reaction is indicated above the lanes. Schematic diagrams of the female-specific (dsx^f) and male-specific (dsx^m) cDNA products are indicated on the left. The markers (M) are ³²P-end-labeled MspI-cleaved pBR322 DNA. (B) Schematic diagram of dsx sex-specific alternative splicing. Boxes represent exons, lines represent introns. The male-specific splice and female-specific splice and polyadenylation site (A) are indicated. The primers used for the RT–PCR (Amrein et al. 1994) are shown schematically above the construct.