Figure 5.

Intra-LA infusion of a DNMT inhibitor interferes with auditory fear memory reconsolidation. (A) Schematic of the behavioral protocol. Rats were trained, followed 24 h later by auditory fear memory retrieval administered in a distinct context, and given intra-LA infusion of either 5-AZA (1 µg in 0.5 µL/side) (n = 7), RG108 (1 µg in 0.5 µL/side) (n = 5), or vehicle (0.5 µL/side) (n = 9) 60 min later. PR-LTM was examined 24 h following the reactivation trial. (B) Post-shock freezing scores in each group immediately after the conditioning trials. (C) Memory reactivation scores in each group during the tone reactivation trial. (D) Auditory fear memory assessed 24 h after reactivation in each group across all 10 tone presentations. (E) Schematic of the behavioral protocol. Rats were trained, followed 24 h later by auditory fear memory retrieval administered in a distinct context, and given intra-LA infusion of either 5-AZA (1 µg in 0.5 µL/side) (n = 7), RG108 (1 µg in 0.5 µL/side) (n = 6), or vehicle (0.5 µL/side) (n = 8) 60 min later. PR-STM was examined 3 h following the reactivation trial. (F) Post-shock freezing scores in each group immediately after the conditioning trials. (G) Memory reactivation scores in each group during the tone reactivation trial. (H) Auditory fear memory assessed at 3 h after memory reactivation in each group across all three tone presentations. (I) Histological verification of cannula placements for rats infused with 5-AZA (gray circles), RG108 (white circles), or vehicle (black circles) and tested for PR-LTM. (J) Histological verification of cannula placements for rats infused with 5-AZA (gray circles), RG108 (white circles), or vehicle (black circles) and tested for PR-STM. (Panels were adapted from Paxinos and Watson [1998] with permission from Elsevier © 1998.)