Table 1.
Plasmid list
| Name
|
Features
|
Parent plasmid
|
Source
|
|---|---|---|---|
| pEApaCla | ApaI, ClaI genomic ENR2 region | pBluescript SK− | this study |
| penr2∷LEU2 | enr2∷LEU2 allele | pEApaCla | this study |
| penr2∷TRP1 | enr2∷TRP1 allele | pEApaCla | this study |
| pEUC | ClaI genomic ENR2 region, URA3, CEN6 | pRS316 and pEL2 | this study |
| BM3280 | Gala4 activation domain fusion with SKP1, LEU2, 2μ | pACT | Mark Johnston (Washington University, St. Louis, Mo) |
| MT839 | CDC53–HAX3, TRP1, CEN6 | pRS314 | Michael Tyers (University of Toronto, Canada) |
| E3a | CDC53, own promoter, URA3, CEN? | YEp24 | Mathias et al., (1995) |
| pCdc53 | CDC53, ADH promoter, TRP1, 2μ | pRS424 | this study |
| pCdc53(1-793) | cdc53(1–793), ADH promoter TRP1 | pRS424 | this study |
| pSJ4101 | CDC4, GAL10 promoter, LEU2 | pSJ101 | Liu et al. (1995) |
| YEp34-1 | CDC34, own promoter, URA3, 2μ | YEp24 | Liu et al. (1995) |
| pYL150 | CDC34, GAL10 promoter, LEU2 | pSJ101 | Liu et al. (1995) |
| pYLB2 | cdc34-2, GAL10 promoter, LEU2 | pSJ101 | Liu et al. (1995) |
| MT798 | CDC53, GAL10 promoter, LEU2 | YEp55 | Mike Tyers |
| p7127 | RUB1, GAL10 promoter, URA3 | pYES2 | this study |
| p7128 | RUB1, RUB1 promoter, URA3, ARS1 CEN4 | pSEYC102 | this study |
| p7147 | HA–RUB1, GAL10 promoter, URA3 | pYES2 | this study |
| pEL2 | ENR2, own promoter, LEU2, 2μ | YEp213 genomic library | this study |
| pMDM152 | GAL1/10 promoter, SIC1 LEU2 CEN4 | Michael Mendenhall (University of Kentucky, Lexington) | |
| pFHE52 | CLN2, MET3 promoter URA3 CEN4 | Duane Hall (University of Wisconsin, Madison) |