Table 1.
Strains and plasmids
| Plasmid
|
Construction or relevant markers
|
Source
|
|---|---|---|
| G4 | M. xanthus cosmid | R. Gill (University of Colorado, Health Sciences, Denver) |
| D10 | M. xanthus cosmid | R. Gill |
| pBGS18 | cloning vector carrying Kmr | Spratt et al. (1986) |
| pBluescript SK+ | cloning vector carrying Apr | Stratagene |
| pTTQ18 | Ptac expression vector | Amersham |
| pPLH323 | cloning vector carrying Kmr and Mx8attP site | P Hartzell (University of Idaho, Moscow) |
| pSWU29 | cloning vector carrying Tcr and Mx8attP site | S. Wu (Stanford University, CA) |
| pPCR-A | 311-bp PCR fragment cloned into pBGS18 | this study |
| pMS131 | control for pMS131, lacks E. coli relA | Singer and Kaiser (1995) |
| pMS132 | E. coli relA gene under Pcar control | Singer and Kaiser (1995) |
| pMS300 | 1.2-kb SacI fragment from pMS302 in pBGS18 | this study |
| pMS302 | 4.8-kb PstI fragment from G4 in pBGS18 | this study |
| pMS320 | 4-kb PstI–NcoI of pMS302 | this study |
| pMS321 | 4-kb PstI–HindIII fragment from pMS320 | this study |
| cloned into pSWU29 (Tcr) | ||
| pMS323 | 1-kb SacI–NcoI fragment from pMS320 into pBGS18 | this study |
| pMS325 | 480-bp EagI (filled in)–XhoI fragment cloned into HindIII (filled in)–XhoI sites of pMS321 (Tcr) | this study |
| pMS350 | 4.8-kb PstI fragment from G4 into pPLH343 | this study |
| pMS379 | 3.2-kb XhoI–PstI fragment into pBGS18 | this study |
| pMS380 | 267-bp BamHI–XhoI PCR fragment | this study |
| cloned into pMS379 | ||
| pMS381 | 3.4-kb BamHI–PstI fragment from pMS380 | this study |
| cloned into pTTQ18–RelA under Ptac control. | ||
| Strains | Relevant genotype | |
| M. xanthus | ||
| DK101 | sglA1 | D. Kaiser (Stanford University, CA) |
| DK527 | DK101 relA527 | Manoil and Kaiser (1980a) |
| DK10528 | DK527 pMS132 | this study |
| DK10529 | DK101 pMS132 | this study |
| DK480 | DK101asgB480 | Kuspa et al. (1986) |
| DK4324 | DK101asgB480 Ω4521 | Kuspa et al. (1986) |
| DK4521 | Ω4521 | Kroos et al. (1986) |
| DK4400 | Ω4400 | Kroos et al. (1986) |
| DK4408 | Ω4408 | Kroos et al. (1986) |
| DK4455 | Ω4455 | Kroos et al. (1986) |
| MS8 | DK101 relA∷pMS302 (tandom duplication of relA region) | this study |
| MS9 | DK527relA∷pMS302 (tandom duplication of relA region) | this study |
| MS10 | DK101 relA∷pMS300 | this study |
| MS11 | DK101 pMS321 | this study |
| MS12 | MS10 pMS321 | this study |
| MS13 | DK527 pMS321 | this study |
| MS15 | DK527 pMS325 | this study |
| MS16 | DK101 pMS325 | this study |
| E. coli | ||
| MG1655 | wild type | B. Bachman (Yale University, New Haven, CT) |
| CF1651 | relAΔ251∷kan | M. Cashel (National Institutes of Health, Bethesda, MD) |
| MS-Ec12 | MG1655 pMS381 | this study |
| MS-Ec51 | CF1651 pMS381 | this study |