Table 3.

A-factor production

Donor strain	In situ productiona		Production in suspensiona
	U/ml	%W.T.	timeb	U/ml	%W.T.c
DK101	480 ± 86	100	3 (hr)	36 ± 3.8	100
(wild type)			6 (hr)	29 ± 3.2	100
			9 (hr)	22 ± 3.1	100
DK480	28 ± 12	6	3 (hr)	0.8 ± 0.3	2
(DK101 asgB)			6 (hr)	1.1 ± 0.4	4
			9 (hr)	1.2 ± 0.4	5
DK527	76 ± 22	16	3 (hr)	2.2 ± 0.8	8
(DK101relA527)			6 (hr)	2.3 ± 0.9	7
			9 (hr)	2.2 ± 0.8	10
MS10	35 ± 08	7	3 (hr)	1.1 ± 0.2	3
(DK101relA∷pMS300)			6 (hr)	0.9 ± 0.4	3
			9 (hr)	0.8 ± 0.4	4
DK10529d	425 ± 73	88	3 (hr)	39 ± 4.8	108
(DK101 pMS132)			6 (hr)	32 ± 4.2	110
			9 (hr)	21 ± 3.1	95
DK10528d	375 ± 76	78	3 (hr)	30 ± 4.6	83
(DK527 pMS132)			6 (hr)	26 ± 4.8	89
			9 (hr)	23 ± 4.8	104

^aValues are shown as units per milliliter of cells (or per milliliter of supernatant recovered from shaken suspensions) at a cell density of 1000 Klett units, prepared as described in Materials and Methods. The values given are an average of three independent experiments. 

^bTime values indicate that the duration cells were cultured in MC7 buffer prior to bioassay of A-factor. 

^c%W.T. activity was calculated by comparing the A-factor activity found in DK480 and DK527 to the amount found in the wild-type strain. Wild-type activity was considered 100% for each time point. 

^dBecause levels of (p)ppGpp adequate for fruiting body development are produced by these strains, experiments were performed in the dark, under conditions that lead to fruiting body formation for these strains.