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Stereotypic read density heterogeneity in ERCC RNA-seq. (A) Traces of relative coverage along ERCC-0002 in two different ENCODE libraries (libraries 7, 8). The pattern is highly reproducible (Pearson's r = 0.96). (B) Average relative coverage along all control RNAs for ERCC spiked in the H. sapiens libraries (libraries 7–50). Dashed lines represent 1 SD around the average across different libraries.
