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. 2011 May 16;193(4):711–725. doi: 10.1083/jcb.201006135

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© 2011 Gudi et al.

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Figure 2. — Centrobin recruitment to the centriole biogenesis site is not dependent on CPAP and CP110. (A, B, C [first and second panels], and D) Centrobin recruitment to the centriole biogenesis site is not inhibited by CPAP and CP110 knockdown. HeLa cells transfected with control, CPAP, or CP110 siRNAs were treated with HU and stained using anti-centrobin, α-tubulin, CPAP, or CP110 antibodies. The percentage of centrobin-positive centrioles is shown in B and D. (C [first and third panels] and E) Knockdown of centrobin inhibits recruitment of CP110. HeLa cells transfected with control or centrobin siRNA and HU were stained using α-tubulin, centrobin, and CP110 antibodies. The percentage of CP110-positive centrioles is shown in E. Histograms are plotted as mean ± SEM (n = 3). The asterisk denotes that the difference is significant, and P < 0.001. Bars, 1 µm.