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. 2011 May 16;193(4):755–767. doi: 10.1083/jcb.201102092

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© 2011 Mao et al.

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Figure 4. — Mitophagy in post-log phase requires Slt2 and Hog1 signaling. (A) MitoPho8Δ60 activity is reduced in slt2Δ, hog1Δ, and hog1Δ slt2Δ mutants in post-log phase mitophagy. Wild-type (KWY20), atg32Δ (KWY22), slt2Δ (KDM1008), hog1Δ (KDM1015), and hog1Δ slt2Δ (KDM1025) cells were cultured in lactate medium to log phase and the cells were grown for another 12, 24, and 36 h. Samples were collected at each time point. The mitoPho8Δ60 assay was performed as described in Materials and methods. Error bars, SD were obtained from three independent repeats. (B) MitoPho8Δ60 activity is reduced in the hog1Δ slt2Δ double mutant in nitrogen starvation–induced mitophagy conditions. The mitoPho8Δ60 assay was performed in wild-type (KWY20), atg32Δ (KWY22), and hog1Δ slt2Δ (KDM1025) cells as in Fig. 1 E.