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. 2011 May 16;193(4):755–767. doi: 10.1083/jcb.201102092

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© 2011 Mao et al.

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Figure 8. — Kinase-dead mutants of Hog1 and Pbs2 have defects in mitophagy. (A) The hog1Δ (KDM1307) or (B) pbs2Δ (KDM1309) cells were transformed with empty vector or a plasmid encoding either (A) wild-type (WT) Hog1 or a kinase-dead mutant (K52R), or (B) with empty vector or a plasmid encoding either wild-type Pbs2 or a kinase-dead mutant (K389R). (A and B) Om45-GFP processing was examined as described in Materials and methods.